rosettesep t cell-depletion kit Search Results


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Rosettesep Cd45 Depletion Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc rosettesep t cell-depletion kit
Rosettesep T Cell Depletion Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc rosettesep kits
Rosettesep Kits, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc anti-cd3 (human cd3+cell depletion kit-rosettesep kit)
Anti Cd3 (Human Cd3+Cell Depletion Kit Rosettesep Kit), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc rosettesep t cell enrichment cocktail
Rosettesep T Cell Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc easysep human cd34 positive selection kit ii
Composition of human HSPC populations varies among graft sources. Flow cytometric analysis of HSPC populations in primary graft tissues. (A) Representative plots of panHLA-class-I + human cells from umbilical cord blood (CB), bone marrow (BM), and G-CSF mobilized peripheral blood (MB), showing the percentage of <t>CD34</t> + cells (red box) in each graft. (B) Aggregated results showing percentage of CD34 + cells detected in individual graft samples. Symbols show results from individual samples of CB (blue), BM (red), or MB (green) tissue, with bars representing the arithmetic means. (C) Representative plots showing flow cytometric staining of CD34 + cells for CD38. (D, E) Aggregated results showing the percentage of cells in the CD38 + (D) or CD38 − (E) gates. (F) Representative plots showing flow cytometric staining of CD34 + CD38 − cells for CD45RA and CD49f. (G–I) Aggregated results showing the percentage of CD34 + CD38 − cells corresponding to uncommitted progenitors (CD45RA + CD49f − , G ), multipotent progenitors (CD45RA − CD49f − , H ), and long-term HSCs (CD45RA − CD49f + , I ). *p < 0.05; **p < 0.01; ***p < 0.001.
Easysep Human Cd34 Positive Selection Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc rosetteseptm human circulating epithelial tumor cell enrichment cocktail #15127
Composition of human HSPC populations varies among graft sources. Flow cytometric analysis of HSPC populations in primary graft tissues. (A) Representative plots of panHLA-class-I + human cells from umbilical cord blood (CB), bone marrow (BM), and G-CSF mobilized peripheral blood (MB), showing the percentage of <t>CD34</t> + cells (red box) in each graft. (B) Aggregated results showing percentage of CD34 + cells detected in individual graft samples. Symbols show results from individual samples of CB (blue), BM (red), or MB (green) tissue, with bars representing the arithmetic means. (C) Representative plots showing flow cytometric staining of CD34 + cells for CD38. (D, E) Aggregated results showing the percentage of cells in the CD38 + (D) or CD38 − (E) gates. (F) Representative plots showing flow cytometric staining of CD34 + CD38 − cells for CD45RA and CD49f. (G–I) Aggregated results showing the percentage of CD34 + CD38 − cells corresponding to uncommitted progenitors (CD45RA + CD49f − , G ), multipotent progenitors (CD45RA − CD49f − , H ), and long-term HSCs (CD45RA − CD49f + , I ). *p < 0.05; **p < 0.01; ***p < 0.001.
Rosetteseptm Human Circulating Epithelial Tumor Cell Enrichment Cocktail #15127, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc enrichment of circulating tumor cells kit rosettesep
Composition of human HSPC populations varies among graft sources. Flow cytometric analysis of HSPC populations in primary graft tissues. (A) Representative plots of panHLA-class-I + human cells from umbilical cord blood (CB), bone marrow (BM), and G-CSF mobilized peripheral blood (MB), showing the percentage of <t>CD34</t> + cells (red box) in each graft. (B) Aggregated results showing percentage of CD34 + cells detected in individual graft samples. Symbols show results from individual samples of CB (blue), BM (red), or MB (green) tissue, with bars representing the arithmetic means. (C) Representative plots showing flow cytometric staining of CD34 + cells for CD38. (D, E) Aggregated results showing the percentage of cells in the CD38 + (D) or CD38 − (E) gates. (F) Representative plots showing flow cytometric staining of CD34 + CD38 − cells for CD45RA and CD49f. (G–I) Aggregated results showing the percentage of CD34 + CD38 − cells corresponding to uncommitted progenitors (CD45RA + CD49f − , G ), multipotent progenitors (CD45RA − CD49f − , H ), and long-term HSCs (CD45RA − CD49f + , I ). *p < 0.05; **p < 0.01; ***p < 0.001.
Enrichment Of Circulating Tumor Cells Kit Rosettesep, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc rosetteseptm monoclonal antibody (mab) kit
Composition of human HSPC populations varies among graft sources. Flow cytometric analysis of HSPC populations in primary graft tissues. (A) Representative plots of panHLA-class-I + human cells from umbilical cord blood (CB), bone marrow (BM), and G-CSF mobilized peripheral blood (MB), showing the percentage of <t>CD34</t> + cells (red box) in each graft. (B) Aggregated results showing percentage of CD34 + cells detected in individual graft samples. Symbols show results from individual samples of CB (blue), BM (red), or MB (green) tissue, with bars representing the arithmetic means. (C) Representative plots showing flow cytometric staining of CD34 + cells for CD38. (D, E) Aggregated results showing the percentage of cells in the CD38 + (D) or CD38 − (E) gates. (F) Representative plots showing flow cytometric staining of CD34 + CD38 − cells for CD45RA and CD49f. (G–I) Aggregated results showing the percentage of CD34 + CD38 − cells corresponding to uncommitted progenitors (CD45RA + CD49f − , G ), multipotent progenitors (CD45RA − CD49f − , H ), and long-term HSCs (CD45RA − CD49f + , I ). *p < 0.05; **p < 0.01; ***p < 0.001.
Rosetteseptm Monoclonal Antibody (Mab) Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc rosettesep b-lymphocyte enrichment kit
Composition of human HSPC populations varies among graft sources. Flow cytometric analysis of HSPC populations in primary graft tissues. (A) Representative plots of panHLA-class-I + human cells from umbilical cord blood (CB), bone marrow (BM), and G-CSF mobilized peripheral blood (MB), showing the percentage of <t>CD34</t> + cells (red box) in each graft. (B) Aggregated results showing percentage of CD34 + cells detected in individual graft samples. Symbols show results from individual samples of CB (blue), BM (red), or MB (green) tissue, with bars representing the arithmetic means. (C) Representative plots showing flow cytometric staining of CD34 + cells for CD38. (D, E) Aggregated results showing the percentage of cells in the CD38 + (D) or CD38 − (E) gates. (F) Representative plots showing flow cytometric staining of CD34 + CD38 − cells for CD45RA and CD49f. (G–I) Aggregated results showing the percentage of CD34 + CD38 − cells corresponding to uncommitted progenitors (CD45RA + CD49f − , G ), multipotent progenitors (CD45RA − CD49f − , H ), and long-term HSCs (CD45RA − CD49f + , I ). *p < 0.05; **p < 0.01; ***p < 0.001.
Rosettesep B Lymphocyte Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Composition of human HSPC populations varies among graft sources. Flow cytometric analysis of HSPC populations in primary graft tissues. (A) Representative plots of panHLA-class-I + human cells from umbilical cord blood (CB), bone marrow (BM), and G-CSF mobilized peripheral blood (MB), showing the percentage of CD34 + cells (red box) in each graft. (B) Aggregated results showing percentage of CD34 + cells detected in individual graft samples. Symbols show results from individual samples of CB (blue), BM (red), or MB (green) tissue, with bars representing the arithmetic means. (C) Representative plots showing flow cytometric staining of CD34 + cells for CD38. (D, E) Aggregated results showing the percentage of cells in the CD38 + (D) or CD38 − (E) gates. (F) Representative plots showing flow cytometric staining of CD34 + CD38 − cells for CD45RA and CD49f. (G–I) Aggregated results showing the percentage of CD34 + CD38 − cells corresponding to uncommitted progenitors (CD45RA + CD49f − , G ), multipotent progenitors (CD45RA − CD49f − , H ), and long-term HSCs (CD45RA − CD49f + , I ). *p < 0.05; **p < 0.01; ***p < 0.001.

Journal: Frontiers in Immunology

Article Title: Different Human Immune Lineage Compositions Are Generated in Non-Conditioned NBSGW Mice Depending on HSPC Source

doi: 10.3389/fimmu.2020.573406

Figure Lengend Snippet: Composition of human HSPC populations varies among graft sources. Flow cytometric analysis of HSPC populations in primary graft tissues. (A) Representative plots of panHLA-class-I + human cells from umbilical cord blood (CB), bone marrow (BM), and G-CSF mobilized peripheral blood (MB), showing the percentage of CD34 + cells (red box) in each graft. (B) Aggregated results showing percentage of CD34 + cells detected in individual graft samples. Symbols show results from individual samples of CB (blue), BM (red), or MB (green) tissue, with bars representing the arithmetic means. (C) Representative plots showing flow cytometric staining of CD34 + cells for CD38. (D, E) Aggregated results showing the percentage of cells in the CD38 + (D) or CD38 − (E) gates. (F) Representative plots showing flow cytometric staining of CD34 + CD38 − cells for CD45RA and CD49f. (G–I) Aggregated results showing the percentage of CD34 + CD38 − cells corresponding to uncommitted progenitors (CD45RA + CD49f − , G ), multipotent progenitors (CD45RA − CD49f − , H ), and long-term HSCs (CD45RA − CD49f + , I ). *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: Where indicated, T cells were depleted using StemCell Technologies RosetteSep T cell-depletion kit (catalog #15661), and HSPCs were isolated using StemCell Technologies EasySep Human CD34 positive selection kit II (catalog #17856), which first depletes lineage positive cells, then positively selects CD34 + cells.

Techniques: Staining

Shift towards short-term HSPC phenotype after transplantation. Flow cytometric analysis was performed to assess the composition of the CD34 + HSPC population within the human compartment in the murine bone marrow at 12 weeks post-transplantation. (A) Representative flow cytometry plots showing identification of human CD34 + population. (B) Plot showing percentage of CD34 + cells detected in bone marrow of mice transplanted with the indicated sources of HSPCs compared to the frequency of total CD34 + cells in the starting graft tissue (Input). (C) Representative plots showing flow cytometric staining of human CD34 + cells in murine bone marrow for CD38. (D, E) Percentage of cells in the CD38 + (D) or CD38 − (E) gates after transplantation compared to the starting graft (Input). (F) Representative plots showing flow cytometric staining of human CD34 + CD38 − cells in the murine bone marrow for CD45RA and CD49f. (G–I) Percentage of CD34 + CD38 − cells corresponding to uncommitted progenitor (CD45RA + CD49f − , G ), multipotent progenitor (CD45RA − CD49f − , H ), and long-term HSC (CD45RA - CD49f + , I ) gates after transplantation compared to the starting graft (Input). Symbols show results from individual mice; bars represent the arithmetic means. *p < 0.05; **p < 0.01; ***p < 0.001.

Journal: Frontiers in Immunology

Article Title: Different Human Immune Lineage Compositions Are Generated in Non-Conditioned NBSGW Mice Depending on HSPC Source

doi: 10.3389/fimmu.2020.573406

Figure Lengend Snippet: Shift towards short-term HSPC phenotype after transplantation. Flow cytometric analysis was performed to assess the composition of the CD34 + HSPC population within the human compartment in the murine bone marrow at 12 weeks post-transplantation. (A) Representative flow cytometry plots showing identification of human CD34 + population. (B) Plot showing percentage of CD34 + cells detected in bone marrow of mice transplanted with the indicated sources of HSPCs compared to the frequency of total CD34 + cells in the starting graft tissue (Input). (C) Representative plots showing flow cytometric staining of human CD34 + cells in murine bone marrow for CD38. (D, E) Percentage of cells in the CD38 + (D) or CD38 − (E) gates after transplantation compared to the starting graft (Input). (F) Representative plots showing flow cytometric staining of human CD34 + CD38 − cells in the murine bone marrow for CD45RA and CD49f. (G–I) Percentage of CD34 + CD38 − cells corresponding to uncommitted progenitor (CD45RA + CD49f − , G ), multipotent progenitor (CD45RA − CD49f − , H ), and long-term HSC (CD45RA - CD49f + , I ) gates after transplantation compared to the starting graft (Input). Symbols show results from individual mice; bars represent the arithmetic means. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: Where indicated, T cells were depleted using StemCell Technologies RosetteSep T cell-depletion kit (catalog #15661), and HSPCs were isolated using StemCell Technologies EasySep Human CD34 positive selection kit II (catalog #17856), which first depletes lineage positive cells, then positively selects CD34 + cells.

Techniques: Transplantation Assay, Flow Cytometry, Staining