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Image Search Results
Journal: Frontiers in Immunology
Article Title: Different Human Immune Lineage Compositions Are Generated in Non-Conditioned NBSGW Mice Depending on HSPC Source
doi: 10.3389/fimmu.2020.573406
Figure Lengend Snippet: Composition of human HSPC populations varies among graft sources. Flow cytometric analysis of HSPC populations in primary graft tissues. (A) Representative plots of panHLA-class-I + human cells from umbilical cord blood (CB), bone marrow (BM), and G-CSF mobilized peripheral blood (MB), showing the percentage of CD34 + cells (red box) in each graft. (B) Aggregated results showing percentage of CD34 + cells detected in individual graft samples. Symbols show results from individual samples of CB (blue), BM (red), or MB (green) tissue, with bars representing the arithmetic means. (C) Representative plots showing flow cytometric staining of CD34 + cells for CD38. (D, E) Aggregated results showing the percentage of cells in the CD38 + (D) or CD38 − (E) gates. (F) Representative plots showing flow cytometric staining of CD34 + CD38 − cells for CD45RA and CD49f. (G–I) Aggregated results showing the percentage of CD34 + CD38 − cells corresponding to uncommitted progenitors (CD45RA + CD49f − , G ), multipotent progenitors (CD45RA − CD49f − , H ), and long-term HSCs (CD45RA − CD49f + , I ). *p < 0.05; **p < 0.01; ***p < 0.001.
Article Snippet: Where indicated, T cells were depleted using StemCell Technologies RosetteSep T cell-depletion kit (catalog #15661), and HSPCs were isolated using
Techniques: Staining
Journal: Frontiers in Immunology
Article Title: Different Human Immune Lineage Compositions Are Generated in Non-Conditioned NBSGW Mice Depending on HSPC Source
doi: 10.3389/fimmu.2020.573406
Figure Lengend Snippet: Shift towards short-term HSPC phenotype after transplantation. Flow cytometric analysis was performed to assess the composition of the CD34 + HSPC population within the human compartment in the murine bone marrow at 12 weeks post-transplantation. (A) Representative flow cytometry plots showing identification of human CD34 + population. (B) Plot showing percentage of CD34 + cells detected in bone marrow of mice transplanted with the indicated sources of HSPCs compared to the frequency of total CD34 + cells in the starting graft tissue (Input). (C) Representative plots showing flow cytometric staining of human CD34 + cells in murine bone marrow for CD38. (D, E) Percentage of cells in the CD38 + (D) or CD38 − (E) gates after transplantation compared to the starting graft (Input). (F) Representative plots showing flow cytometric staining of human CD34 + CD38 − cells in the murine bone marrow for CD45RA and CD49f. (G–I) Percentage of CD34 + CD38 − cells corresponding to uncommitted progenitor (CD45RA + CD49f − , G ), multipotent progenitor (CD45RA − CD49f − , H ), and long-term HSC (CD45RA - CD49f + , I ) gates after transplantation compared to the starting graft (Input). Symbols show results from individual mice; bars represent the arithmetic means. *p < 0.05; **p < 0.01; ***p < 0.001.
Article Snippet: Where indicated, T cells were depleted using StemCell Technologies RosetteSep T cell-depletion kit (catalog #15661), and HSPCs were isolated using
Techniques: Transplantation Assay, Flow Cytometry, Staining